C7930
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 | Immunofluorescence Human Tonsil stained with Anti-CD3, T Cell (Cat. No. C7930) at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen (Cat. No. P8825) at a 1:500 dilution (green). Counterstained with DAPI. From Pamela Wirth, Plastic Surgery Department, Vanderbilt University, Nashville, TN. |
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C7930
Sigma
Anti-CD3, T Cell antibody produced in rabbit
whole antiserum
| MDL number: | MFCD00162554 |
Description
| Immunogen | synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method. |
| Specificity | The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin's solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species.1 |
Properties
| antibody form | whole antiserum |
| clone | (polyclonal) |
| mol wt | antigen mol wt 20 kDa (bovine and swine) |
| contains | 15 mM sodium azide |
| species reactivity | pig, human, bovine, canine, mouse, Psammomys (sand rat), sheep, rat, feline, chicken |
| application(s) | immunoblotting: 1:200 using mouse thymus extract |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections | |
| Gene Information | human ... CD3E(916) |
| rat ... Cd3e(315609) | |
| mouse ... Cd3e(12501) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| Cited Reference | 1. Kurviz, E., et al. Proc. 8th Int. Cong. of Immuno., 7, (1992) |
| reference | Mason, D.Y., et al. J. Clin. Pathol. 42, 1194, (1989) Abstract |
| Martin, M., et al., Spontaneous mammary tumors differ widely in their inherent sensitivity to adoptively transferred T cells. Cancer Res. 67, 6442-6450, (2007) Abstract | |
| Fed’kiv, O., et al., Precocious gut maturation and immune cell expansion by single dose feeding the lectin phytohaemagglutinin to suckling rats. Br. J. Nutr. 46, 1631-1640, (2008) |
